Detailed information of TA system TA208249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 678593..679070
• Replicon: chromosome
• Accession: NZ_CP077863
• Organism: Staphylococcus aureus strain 371

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KU524_RS03225
• Protein ID: WP_000052076.1
• Coordinates: 678882..679070 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KU524_RS03220
• Protein ID: WP_001059690.1
• Coordinates: 678593..678853 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU524_RS03185 (673986)	673986..674702	-	717	WP_000150029.1	type I 3-dehydroquinate dehydratase	-
KU524_RS03190 (674850)	674850..675272	+	423	WP_000974455.1	organic hydroperoxide resistance protein	-
KU524_RS03195 (675671)	675671..676165	+	495	WP_000569881.1	GNAT family N-acetyltransferase	-
KU524_RS03200 (676321)	676321..676938	+	618	WP_000255554.1	LysE/ArgO family amino acid transporter	-
KU524_RS03205 (677011)	677011..677592	-	582	WP_001140697.1	histidine phosphatase family protein	-
KU524_RS03210 (677816)	677816..678052	+	237	WP_000368687.1	hypothetical protein	-
KU524_RS03215 (678049)	678049..678252	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KU524_RS03220 (678593)	678593..678853	+	261	WP_001059690.1	hypothetical protein	Antitoxin
KU524_RS03225 (678882)	678882..679070	+	189	WP_000052076.1	hypothetical protein	Toxin
KU524_RS03230 (679258)	679258..679827	+	570	WP_001106657.1	lipoprotein N-acylation protein LnsA	-
KU524_RS03235 (679915)	679915..680199	+	285	WP_001004397.1	hypothetical protein	-
KU524_RS03240 (680261)	680261..680479	+	219	WP_000005888.1	hypothetical protein	-
KU524_RS03245 (680978)	680978..681178	-	201	WP_001059082.1	cold-shock protein	-
KU524_RS03250 (681535)	681535..682221	-	687	WP_001823812.1	thermonuclease family protein	-
KU524_RS03255 (682562)	682562..683032	-	471	WP_000824462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	seb / selq / selk	651043..684390	33347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T208249 WP_000052076.1 NZ_CP077863:678882-679070 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T208249 NZ_CP103605:383096-383443 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT208249 WP_001059690.1 NZ_CP077863:678593-678853 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT208249 NZ_CP103605:383443-383742 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56