Detailed information of TA system TA208239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 673899..674428
• Replicon: chromosome
• Accession: NZ_CP077861
• Organism: Staphylococcus aureus strain 372

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU510_RS03445
• Protein ID: WP_000621175.1
• Coordinates: 673899..674261 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU510_RS03450
• Protein ID: WP_000948331.1
• Coordinates: 674258..674428 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU510_RS03425 (670878)	670878..671648	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU510_RS03430 (671623)	671623..672102	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU510_RS03435 (672104)	672104..672430	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU510_RS03440 (672549)	672549..673550	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU510_RS03445 (673899)	673899..674261	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU510_RS03450 (674258)	674258..674428	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU510_RS03455 (674513)	674513..675661	-	1149	WP_001280702.1	alanine racemase	-
KU510_RS03460 (675727)	675727..676086	-	360	WP_000581194.1	holo-ACP synthase	-
KU510_RS03465 (676090)	676090..676581	-	492	WP_001205915.1	PH domain-containing protein	-
KU510_RS03470 (676568)	676568..678151	-	1584	WP_001294642.1	PH domain-containing protein	-
KU510_RS03475 (678144)	678144..678623	-	480	WP_001287083.1	hypothetical protein	-
KU510_RS03480 (678832)	678832..679392	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208239 WP_000621175.1 NZ_CP077861:c674261-673899 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208239 NZ_CP103596:3648709-3648987 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208239 WP_000948331.1 NZ_CP077861:c674428-674258 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208239 NZ_CP103596:3648446-3648706 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1