Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1058525..1059039 Replicon chromosome
Accession NZ_CP077860
Organism Staphylococcus aureus strain AC4

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU521_RS05325 Protein ID WP_000581792.1
Coordinates 1058525..1058734 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU521_RS05330 Protein ID WP_000138932.1
Coordinates 1058746..1059039 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU521_RS05305 (1054033) 1054033..1055391 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KU521_RS05310 (1055406) 1055406..1056476 - 1071 WP_225798614.1 D-alanine--D-alanine ligase -
KU521_RS05315 (1056794) 1056794..1057996 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU521_RS05320 (1058260) 1058260..1058397 - 138 WP_000828354.1 Lmo0850 family protein -
KU521_RS05325 (1058525) 1058525..1058734 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU521_RS05330 (1058746) 1058746..1059039 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU521_RS05335 (1059205) 1059205..1060689 + 1485 WP_000571549.1 cardiolipin synthase -
KU521_RS05340 (1060717) 1060717..1061364 + 648 WP_001187630.1 HD domain-containing protein -
KU521_RS05345 (1062177) 1062177..1063049 - 873 WP_000725802.1 membrane protein insertase YidC -
KU521_RS05350 (1063135) 1063135..1063776 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208227 WP_000581792.1 NZ_CP077860:c1058734-1058525 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208227 NZ_CP103596:1321033-1321431 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208227 WP_000138932.1 NZ_CP077860:c1059039-1058746 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208227 NZ_CP103596:1320806-1321033 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References