Detailed information of TA system TA208226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1037266..1037795
• Replicon: chromosome
• Accession: NZ_CP077860
• Organism: Staphylococcus aureus strain AC4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU521_RS05225
• Protein ID: WP_000621175.1
• Coordinates: 1037266..1037628 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU521_RS05230
• Protein ID: WP_000948331.1
• Coordinates: 1037625..1037795 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU521_RS05205 (1034244)	1034244..1035014	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
KU521_RS05210 (1034989)	1034989..1035468	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU521_RS05215 (1035470)	1035470..1035796	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU521_RS05220 (1035915)	1035915..1036916	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU521_RS05225 (1037266)	1037266..1037628	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU521_RS05230 (1037625)	1037625..1037795	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU521_RS05235 (1037880)	1037880..1039028	-	1149	WP_001281154.1	alanine racemase	-
KU521_RS05240 (1039094)	1039094..1039453	-	360	WP_000581197.1	holo-ACP synthase	-
KU521_RS05245 (1039457)	1039457..1039948	-	492	WP_001205912.1	PH domain-containing protein	-
KU521_RS05250 (1039935)	1039935..1041518	-	1584	WP_001294626.1	PH domain-containing protein	-
KU521_RS05255 (1041511)	1041511..1041990	-	480	WP_001287087.1	hypothetical protein	-
KU521_RS05260 (1042198)	1042198..1042758	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208226 WP_000621175.1 NZ_CP077860:c1037628-1037266 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208226 NZ_CP103596:1037389-1037724 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208226 WP_000948331.1 NZ_CP077860:c1037795-1037625 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208226 NZ_CP103596:1037141-1037389 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1