Detailed information of TA system TA208221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 838566..839342
• Replicon: chromosome
• Accession: NZ_CP077860
• Organism: Staphylococcus aureus strain AC4

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KU521_RS03980
• Protein ID: WP_000031108.1
• Coordinates: 838566..838718 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: KU521_RS03985
• Protein ID: WP_001251224.1
• Coordinates: 838743..839342 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU521_RS03965 (834768)	834768..835589	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
KU521_RS03970 (836042)	836042..837427	-	1386	WP_000116228.1	class II fumarate hydratase	-
KU521_RS03975 (837623)	837623..838018	-	396	WP_000901023.1	hypothetical protein	-
KU521_RS03980 (838566)	838566..838718	-	153	WP_000031108.1	SAS053 family protein	Toxin
KU521_RS03985 (838743)	838743..839342	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
KU521_RS03990 (839501)	839501..839971	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KU521_RS03995 (839976)	839976..841103	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
KU521_RS04000 (841254)	841254..841976	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KU521_RS04005 (841969)	841969..843426	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T208221 WP_000031108.1 NZ_CP077860:c838718-838566 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T208221 NZ_CP103593:c118964-118659 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT208221 WP_001251224.1 NZ_CP077860:c839342-838743 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT208221 NZ_CP103593:c119184-118966 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4