Detailed information of TA system TA208214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2567271..2567800
• Replicon: chromosome
• Accession: NZ_CP077859
• Organism: Staphylococcus aureus strain AC5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU537_RS12500
• Protein ID: WP_000621175.1
• Coordinates: 2567438..2567800 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU537_RS12495
• Protein ID: WP_000948331.1
• Coordinates: 2567271..2567441 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU537_RS12465 (2562308)	2562308..2562868	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU537_RS12470 (2563076)	2563076..2563555	+	480	WP_001287087.1	hypothetical protein	-
KU537_RS12475 (2563548)	2563548..2565131	+	1584	WP_001294626.1	PH domain-containing protein	-
KU537_RS12480 (2565118)	2565118..2565609	+	492	WP_001205912.1	PH domain-containing protein	-
KU537_RS12485 (2565613)	2565613..2565972	+	360	WP_000581197.1	holo-ACP synthase	-
KU537_RS12490 (2566038)	2566038..2567186	+	1149	WP_001281154.1	alanine racemase	-
KU537_RS12495 (2567271)	2567271..2567441	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU537_RS12500 (2567438)	2567438..2567800	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU537_RS12505 (2568150)	2568150..2569151	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU537_RS12510 (2569270)	2569270..2569596	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU537_RS12515 (2569598)	2569598..2570077	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU537_RS12520 (2570052)	2570052..2570822	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208214 WP_000621175.1 NZ_CP077859:2567438-2567800 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208214 NZ_CP103592:c4160722-4160438 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208214 WP_000948331.1 NZ_CP077859:2567271-2567441 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208214 NZ_CP103592:c4160958-4160725 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1