Detailed information of TA system TA208197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2584630..2585159
• Replicon: chromosome
• Accession: NZ_CP077858
• Organism: Staphylococcus aureus strain AC6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU543_RS12705
• Protein ID: WP_000621175.1
• Coordinates: 2584797..2585159 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU543_RS12700
• Protein ID: WP_000948331.1
• Coordinates: 2584630..2584800 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU543_RS12670 (2579667)	2579667..2580227	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU543_RS12675 (2580435)	2580435..2580914	+	480	WP_001287087.1	hypothetical protein	-
KU543_RS12680 (2580907)	2580907..2582490	+	1584	WP_001294626.1	PH domain-containing protein	-
KU543_RS12685 (2582477)	2582477..2582968	+	492	WP_001205912.1	PH domain-containing protein	-
KU543_RS12690 (2582972)	2582972..2583331	+	360	WP_000581197.1	holo-ACP synthase	-
KU543_RS12695 (2583397)	2583397..2584545	+	1149	WP_001281154.1	alanine racemase	-
KU543_RS12700 (2584630)	2584630..2584800	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU543_RS12705 (2584797)	2584797..2585159	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU543_RS12710 (2585509)	2585509..2586510	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU543_RS12715 (2586629)	2586629..2586955	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU543_RS12720 (2586957)	2586957..2587436	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU543_RS12725 (2587411)	2587411..2588181	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208197 WP_000621175.1 NZ_CP077858:2584797-2585159 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208197 NZ_CP103591:80858-81163 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208197 WP_000948331.1 NZ_CP077858:2584630-2584800 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208197 NZ_CP103591:80638-80856 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1