Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 554741..555359 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C_RS02655 Protein ID WP_001291435.1
Coordinates 554741..554959 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C_RS02660 Protein ID WP_000344800.1
Coordinates 554985..555359 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS02625 550029..550601 + 573 WP_000779831.1 YbaY family lipoprotein -
C_RS02630 550632..550943 - 312 WP_000409908.1 MGMT family protein -
C_RS02635 551322..551675 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C_RS02640 551717..553267 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C_RS02645 553431..553901 - 471 WP_001304825.1 YlaC family protein -
C_RS02650 554017..554568 - 552 WP_000102539.1 maltose O-acetyltransferase -
C_RS02655 554741..554959 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C_RS02660 554985..555359 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C_RS02665 555904..559053 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C_RS02670 559076..560269 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T20819 WP_001291435.1 NC_004431:c554959-554741 [Escherichia coli CFT073]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T20819 NC_004431:c554959-554741 [Escherichia coli CFT073]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT20819 WP_000344800.1 NC_004431:c555359-554985 [Escherichia coli CFT073]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT20819 NC_004431:c555359-554985 [Escherichia coli CFT073]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References