Detailed information of TA system TA208179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2584150..2584679
• Replicon: chromosome
• Accession: NZ_CP077857
• Organism: Staphylococcus aureus strain L4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU533_RS12700
• Protein ID: WP_000621175.1
• Coordinates: 2584317..2584679 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU533_RS12695
• Protein ID: WP_000948331.1
• Coordinates: 2584150..2584320 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU533_RS12665 (2579187)	2579187..2579747	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU533_RS12670 (2579955)	2579955..2580434	+	480	WP_001287087.1	hypothetical protein	-
KU533_RS12675 (2580427)	2580427..2582010	+	1584	WP_001294626.1	PH domain-containing protein	-
KU533_RS12680 (2581997)	2581997..2582488	+	492	WP_001205912.1	PH domain-containing protein	-
KU533_RS12685 (2582492)	2582492..2582851	+	360	WP_000581197.1	holo-ACP synthase	-
KU533_RS12690 (2582917)	2582917..2584065	+	1149	WP_001281154.1	alanine racemase	-
KU533_RS12695 (2584150)	2584150..2584320	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU533_RS12700 (2584317)	2584317..2584679	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU533_RS12705 (2585029)	2585029..2586030	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU533_RS12710 (2586149)	2586149..2586475	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU533_RS12715 (2586477)	2586477..2586956	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU533_RS12720 (2586931)	2586931..2587701	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208179 WP_000621175.1 NZ_CP077857:2584317-2584679 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208179 NZ_CP103589:1111855-1112190 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208179 WP_000948331.1 NZ_CP077857:2584150-2584320 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208179 NZ_CP103589:1111607-1111855 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1