Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53019..53569 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag C_RS00280 Protein ID WP_001160966.1
Coordinates 53255..53569 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C_RS00275 Protein ID WP_000125566.1
Coordinates 53019..53252 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS00250 48092..48379 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C_RS00255 48438..49769 + 1332 WP_001183207.1 MFS transporter -
C_RS00260 49877..50407 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C_RS00265 50400..52262 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
C_RS00270 52454..52933 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
C_RS00275 53019..53252 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C_RS00280 53255..53569 + 315 WP_001160966.1 CcdB family protein Toxin
C_RS00285 53566..54414 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C_RS00290 54421..54798 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C_RS00295 54801..55622 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C_RS00300 55619..56608 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C_RS00305 56608..57894 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T20817 WP_001160966.1 NC_004431:53255-53569 [Escherichia coli CFT073]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T20817 NC_004431:53255-53569 [Escherichia coli CFT073]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT20817 WP_000125566.1 NC_004431:53019-53252 [Escherichia coli CFT073]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT20817 NC_004431:53019-53252 [Escherichia coli CFT073]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References