Detailed information of TA system TA208161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2572103..2572632
• Replicon: chromosome
• Accession: NZ_CP077856
• Organism: Staphylococcus aureus strain L5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU529_RS12530
• Protein ID: WP_000621175.1
• Coordinates: 2572270..2572632 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU529_RS12525
• Protein ID: WP_000948331.1
• Coordinates: 2572103..2572273 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU529_RS12495 (2567140)	2567140..2567700	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU529_RS12500 (2567908)	2567908..2568387	+	480	WP_001287087.1	hypothetical protein	-
KU529_RS12505 (2568380)	2568380..2569963	+	1584	WP_001294626.1	PH domain-containing protein	-
KU529_RS12510 (2569950)	2569950..2570441	+	492	WP_001205912.1	PH domain-containing protein	-
KU529_RS12515 (2570445)	2570445..2570804	+	360	WP_000581197.1	holo-ACP synthase	-
KU529_RS12520 (2570870)	2570870..2572018	+	1149	WP_001281154.1	alanine racemase	-
KU529_RS12525 (2572103)	2572103..2572273	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU529_RS12530 (2572270)	2572270..2572632	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU529_RS12535 (2572982)	2572982..2573983	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU529_RS12540 (2574102)	2574102..2574428	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU529_RS12545 (2574430)	2574430..2574909	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU529_RS12550 (2574884)	2574884..2575654	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208161 WP_000621175.1 NZ_CP077856:2572270-2572632 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208161 NZ_CP103584:36253-36771 [Klebsiella pneumoniae]
GTGAACACGTATGATATTGAACCAATACAGAAAGATTTTCCATACGACATTAGCTCCTTCGACTGCGGTAACGAGGCGCT
AAATTTATTCCTGTCAGAACATCTTCAAAGACAGGACGAAAAGCAGATTCTGCGTGGATACGTATATCTTGAGTATGGAT
CTGGCTTACCAAAAGTGCTTGGCTTCTTTACTTTGGCCGGGGGGTCGTTTGAGCGAAGTAGATTTCCATCCAAAAGCCAG
CAGAAGAAAATTCCTTATACCAATACCCCATGCATAATCCTGGGCCGACTGGCTGTCGACAAGACAGTCCAGCGACAAGG
CATTGGTGAAATGCTGGTGGTCGAGGCAGCAAAAGTGGTTCTTCAAGCAGCTAACGCGATAGGTCTTCATGGTATGTTTG
TTGACGCTAAGAATGAATCTGCTTGTAAATTTTATGAAAATTTAGGGTTTACTAGGTTGATTGGTGATAATGAAAACCTA
TTCTTTTACCCTACATCCAAAATTGCACAGCTCCCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208161 WP_000948331.1 NZ_CP077856:2572103-2572273 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208161 NZ_CP103584:35976-36248 [Klebsiella pneumoniae]
ATGCCCACTTTGAAAAAAGAACGTATCGATATACGACTGACCGATGAAGCTAAGTCAGTCATTGAGGAAGCGGCGATCTT
GTCCAATCTAAGCGTGAGCCAGTTTGTGATAAGTAGCGCAGCAGAAAGAGCCACAGAGGTGATTGAGCAGCATCGCAGAA
TCGTTTTGTGCGAAGATTCCTGGAAATTGGTGATGGATGCCATCAACAACCCAGATCAACCGAATGACAGAATGAAGCGA
GCTGCTGAGCGCCTTAAAAATTTAAGGACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1