Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2550859..2551373 Replicon chromosome
Accession NZ_CP077856
Organism Staphylococcus aureus strain L5

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU529_RS12430 Protein ID WP_000581792.1
Coordinates 2551164..2551373 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU529_RS12425 Protein ID WP_000138932.1
Coordinates 2550859..2551152 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU529_RS12405 (2546122) 2546122..2546763 + 642 WP_000483153.1 thiamine phosphate synthase -
KU529_RS12410 (2546849) 2546849..2547721 + 873 WP_000725802.1 membrane protein insertase YidC -
KU529_RS12415 (2548534) 2548534..2549181 - 648 WP_001187630.1 HD domain-containing protein -
KU529_RS12420 (2549209) 2549209..2550693 - 1485 WP_000571549.1 cardiolipin synthase -
KU529_RS12425 (2550859) 2550859..2551152 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU529_RS12430 (2551164) 2551164..2551373 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU529_RS12435 (2551501) 2551501..2551638 + 138 WP_000828354.1 Lmo0850 family protein -
KU529_RS12440 (2551902) 2551902..2553104 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU529_RS12445 (2553422) 2553422..2554492 + 1071 WP_225798614.1 D-alanine--D-alanine ligase -
KU529_RS12450 (2554507) 2554507..2555865 + 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208160 WP_000581792.1 NZ_CP077856:2551164-2551373 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208160 NZ_CP103583:11346-11762 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208160 WP_000138932.1 NZ_CP077856:2550859-2551152 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208160 NZ_CP103583:11119-11349 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References