Detailed information of TA system TA208135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 329656..330185
• Replicon: chromosome
• Accession: NZ_CP077855
• Organism: Staphylococcus aureus strain L6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU520_RS01720
• Protein ID: WP_000621175.1
• Coordinates: 329823..330185 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU520_RS01715
• Protein ID: WP_000948331.1
• Coordinates: 329656..329826 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU520_RS01685 (324693)	324693..325253	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU520_RS01690 (325461)	325461..325940	+	480	WP_001287087.1	hypothetical protein	-
KU520_RS01695 (325933)	325933..327516	+	1584	WP_001294626.1	PH domain-containing protein	-
KU520_RS01700 (327503)	327503..327994	+	492	WP_001205912.1	PH domain-containing protein	-
KU520_RS01705 (327998)	327998..328357	+	360	WP_000581197.1	holo-ACP synthase	-
KU520_RS01710 (328423)	328423..329571	+	1149	WP_001281154.1	alanine racemase	-
KU520_RS01715 (329656)	329656..329826	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU520_RS01720 (329823)	329823..330185	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU520_RS01725 (330535)	330535..331536	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU520_RS01730 (331655)	331655..331981	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU520_RS01735 (331983)	331983..332462	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU520_RS01740 (332437)	332437..333207	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208135 WP_000621175.1 NZ_CP077855:329823-330185 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208135 NZ_CP103577:c4344869-4344498 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208135 WP_000948331.1 NZ_CP077855:329656-329826 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208135 NZ_CP103577:c4345114-4344872 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATTGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAGCCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1