Detailed information of TA system TA020813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1391052..1392298
• Replicon: chromosome
• Accession: NC_004368
• Organism: Streptococcus agalactiae NEM316

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: Q8E4Q8
• Locus tag: GBS_RS07085
• Protein ID: WP_000170758.1
• Coordinates: 1391052..1391822 (-)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: Q8E4Q7
• Locus tag: GBS_RS07090
• Protein ID: WP_000578715.1
• Coordinates: 1391822..1392298 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBS_RS07070	1386083..1386442	-	360	WP_011074740.1	hypothetical protein	-
GBS_RS07075	1386777..1388795	-	2019	WP_000222644.1	ATP-dependent helicase	-
GBS_RS07080	1388799..1391039	-	2241	WP_000281027.1	AAA family ATPase	-
GBS_RS07085	1391052..1391822	-	771	WP_000170758.1	zeta toxin family protein	Toxin
GBS_RS07090	1391822..1392298	-	477	WP_000578715.1	helix-turn-helix domain-containing protein	Antitoxin
GBS_RS07095	1392368..1392658	-	291	WP_001210630.1	hypothetical protein	-
GBS_RS07100	1392705..1393094	-	390	WP_000132515.1	hypothetical protein	-
GBS_RS07105	1393091..1393321	-	231	WP_000110918.1	hypothetical protein	-
GBS_RS07110	1393376..1394461	-	1086	WP_000161120.1	toprim domain-containing protein	-
GBS_RS07115	1394502..1395140	-	639	WP_000932868.1	hypothetical protein	-
GBS_RS07120	1395305..1395604	-	300	WP_001147539.1	hypothetical protein	-
GBS_RS07125	1395615..1395914	-	300	WP_000084342.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	lmb / scpB	1312217..1405972	93755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29353.47 Da
• Isoelectric Point: 8.7794

>T20813 WP_000170758.1 NC_004368:c1391822-1391052 [Streptococcus agalactiae NEM316]
MTLAEYSEAEFQKALRRNIRALTRGKTISSTPKAVLLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSFRSQHPNYLALQE
EYGKYSVDYTKDFAGKMVEHLIGGLSKQSYHLLIEGTLRTTEVPRKTAQLLKSRGYQVSLALIATKPELSYLSTLIRYEE
LYAIDPNQARATPKEYHDGIVEHLVDNLRELENDKLFNRIQIYQRDRHCVYDSETDKGIAAEVLQECLFGKWSKVEEEML
RVGQERLRELSMRTPK

Nucleotide

• Length: 771 bp

>T20813 NC_004368:c1391822-1391052 [Streptococcus agalactiae NEM316]
ATGACATTAGCAGAATATAGTGAGGCGGAGTTTCAAAAGGCTTTAAGGCGTAATATTCGTGCTTTAACCCGTGGCAAAAC
TATTTCCTCAACTCCCAAAGCAGTTCTGCTTGGTGGTCAAAGTGGAGCTGGAAAAACGACCATTCATCGAATCAAGCAAA
AGGAATTTCAAGGGAATATCATCATTATTGACGGAGATAGCTTCCGTTCGCAGCATCCAAATTATCTGGCTTTGCAGGAA
GAGTATGGCAAATATAGCGTTGATTATACCAAAGATTTTGCGGGGAAGATGGTTGAACACCTCATTGGTGGACTCAGCAA
ACAAAGCTATCATTTATTAATTGAGGGAACACTGAGAACAACAGAAGTCCCTCGAAAAACAGCTCAATTATTAAAATCAA
GAGGCTATCAAGTATCACTTGCGCTCATCGCAACAAAGCCAGAACTGTCCTATCTTAGCACCTTGATACGCTATGAAGAA
CTTTATGCCATAGATCCTAATCAAGCCAGAGCAACACCAAAAGAATATCATGATGGGATTGTGGAACACTTGGTTGATAA
TCTACGAGAGTTAGAAAATGATAAACTTTTTAACCGGATTCAAATCTATCAAAGAGATAGACATTGTGTCTATGATTCTG
AAACTGATAAAGGCATAGCTGCAGAAGTGCTTCAAGAATGTCTCTTTGGGAAATGGAGTAAGGTAGAGGAGGAGATGTTG
AGGGTGGGACAGGAACGGTTGAGGGAGTTAAGTATGAGAACCCCAAAATAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18145.47 Da
• Isoelectric Point: 4.4655

>AT20813 WP_000578715.1 NC_004368:c1392298-1391822 [Streptococcus agalactiae NEM316]
MIGDNIKSLRRTHDLTQPEFAKIIGISRNSLSRYENGTSSVSTELIDRICQKFNVSYVDIVGEDKMMTPVEDYQLTIKIE
VLKERGSAILSRLYRHQDEQGIAFDDESNPWILISDDVADLVNTKIYLVDTFEELERYNGYLDGIERMLNLANHQVVA

Nucleotide

• Length: 477 bp

>AT20813 NC_004368:c1392298-1391822 [Streptococcus agalactiae NEM316]
ATGATTGGAGATAATATTAAGTCCTTACGACGAACACATGATTTAACGCAGCCAGAGTTCGCAAAAATTATTGGTATTTC
AAGGAATAGTCTTAGTCGTTATGAAAATGGAACCAGCTCCGTTTCAACCGAACTCATTGATCGGATTTGTCAGAAATTTA
ATGTGTCTTATGTCGATATTGTTGGAGAAGATAAGATGATGACACCCGTAGAAGATTATCAGCTGACTATAAAAATTGAA
GTACTGAAAGAACGAGGTTCTGCCATTTTGTCAAGGCTGTACCGCCATCAAGATGAGCAAGGGATTGCATTTGACGATGA
ATCGAATCCGTGGATTTTGATAAGTGATGATGTAGCAGATTTAGTGAATACCAAGATTTATCTTGTCGATACCTTTGAAG
AGTTAGAGCGTTACAATGGCTATTTAGATGGTATTGAGCGGATGTTAAACCTTGCCAATCACCAGGTGGTGGCCTAA

Structures

Toxin

• AlphaFold DB: Q8E4Q8

Antitoxin

• AlphaFold DB: Q8E4Q7