Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 566235..566758 Replicon chromosome
Accession NZ_CP077854
Organism Staphylococcus aureus strain N4

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag KU508_RS03040 Protein ID WP_000074049.1
Coordinates 566235..566501 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag KU508_RS03045 Protein ID WP_000587616.1
Coordinates 566501..566758 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU508_RS03020 (561542) 561542..562729 - 1188 WP_000026189.1 MFS transporter -
KU508_RS03025 (563125) 563125..563901 - 777 WP_000072149.1 iron export ABC transporter permease subunit FetB -
KU508_RS03030 (563894) 563894..564556 - 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
KU508_RS03035 (564804) 564804..565880 - 1077 WP_001076673.1 M42 family metallopeptidase -
KU508_RS03040 (566235) 566235..566501 - 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
KU508_RS03045 (566501) 566501..566758 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KU508_RS03050 (567117) 567117..567572 + 456 WP_000732076.1 DUF1307 domain-containing protein -
KU508_RS03055 (567740) 567740..569317 - 1578 WP_000143494.1 FMN-binding glutamate synthase family protein -
KU508_RS03060 (569512) 569512..570261 + 750 WP_000072461.1 CPBP family lipoprotein N-acylation protein LnsB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T208125 WP_000074049.1 NZ_CP077854:c566501-566235 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T208125 NZ_CP103572:78693-78968 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAAGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT208125 WP_000587616.1 NZ_CP077854:c566758-566501 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT208125 NZ_CP103572:78409-78693 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References