Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 191292..191806 Replicon chromosome
Accession NZ_CP077854
Organism Staphylococcus aureus strain N4

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU508_RS01095 Protein ID WP_000581792.1
Coordinates 191292..191501 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU508_RS01100 Protein ID WP_000138932.1
Coordinates 191513..191806 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU508_RS01075 (186800) 186800..188158 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KU508_RS01080 (188173) 188173..189243 - 1071 WP_225798614.1 D-alanine--D-alanine ligase -
KU508_RS01085 (189561) 189561..190763 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU508_RS01090 (191027) 191027..191164 - 138 WP_000828354.1 Lmo0850 family protein -
KU508_RS01095 (191292) 191292..191501 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU508_RS01100 (191513) 191513..191806 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU508_RS01105 (191972) 191972..193456 + 1485 WP_000571549.1 cardiolipin synthase -
KU508_RS01110 (193484) 193484..194131 + 648 WP_001187630.1 HD domain-containing protein -
KU508_RS01115 (194944) 194944..195816 - 873 WP_000725802.1 membrane protein insertase YidC -
KU508_RS01120 (195902) 195902..196543 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208119 WP_000581792.1 NZ_CP077854:c191501-191292 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208119 NZ_CP103570:c4133267-4132917 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208119 WP_000138932.1 NZ_CP077854:c191806-191513 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208119 NZ_CP103570:c4133512-4133261 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References