Detailed information of TA system TA208118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 170033..170562
• Replicon: chromosome
• Accession: NZ_CP077854
• Organism: Staphylococcus aureus strain N4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU508_RS00995
• Protein ID: WP_000621175.1
• Coordinates: 170033..170395 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU508_RS01000
• Protein ID: WP_000948331.1
• Coordinates: 170392..170562 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU508_RS00975 (167011)	167011..167781	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
KU508_RS00980 (167756)	167756..168235	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU508_RS00985 (168237)	168237..168563	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU508_RS00990 (168682)	168682..169683	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU508_RS00995 (170033)	170033..170395	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU508_RS01000 (170392)	170392..170562	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU508_RS01005 (170647)	170647..171795	-	1149	WP_001281154.1	alanine racemase	-
KU508_RS01010 (171861)	171861..172220	-	360	WP_000581197.1	holo-ACP synthase	-
KU508_RS01015 (172224)	172224..172715	-	492	WP_001205912.1	PH domain-containing protein	-
KU508_RS01020 (172702)	172702..174285	-	1584	WP_001294626.1	PH domain-containing protein	-
KU508_RS01025 (174278)	174278..174757	-	480	WP_001287087.1	hypothetical protein	-
KU508_RS01030 (174965)	174965..175525	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208118 WP_000621175.1 NZ_CP077854:c170395-170033 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208118 NZ_CP103570:c4018011-4017637 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGACTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208118 WP_000948331.1 NZ_CP077854:c170562-170392 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208118 NZ_CP103570:c4018469-4018101 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1