Detailed information of TA system TA208109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2585128..2585657
• Replicon: chromosome
• Accession: NZ_CP077853
• Organism: Staphylococcus aureus strain N5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU499_RS12710
• Protein ID: WP_000621175.1
• Coordinates: 2585295..2585657 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU499_RS12705
• Protein ID: WP_000948331.1
• Coordinates: 2585128..2585298 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU499_RS12675 (2580165)	2580165..2580725	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU499_RS12680 (2580933)	2580933..2581412	+	480	WP_001287087.1	hypothetical protein	-
KU499_RS12685 (2581405)	2581405..2582988	+	1584	WP_001294626.1	PH domain-containing protein	-
KU499_RS12690 (2582975)	2582975..2583466	+	492	WP_001205912.1	PH domain-containing protein	-
KU499_RS12695 (2583470)	2583470..2583829	+	360	WP_000581197.1	holo-ACP synthase	-
KU499_RS12700 (2583895)	2583895..2585043	+	1149	WP_001281154.1	alanine racemase	-
KU499_RS12705 (2585128)	2585128..2585298	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU499_RS12710 (2585295)	2585295..2585657	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU499_RS12715 (2586007)	2586007..2587008	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU499_RS12720 (2587127)	2587127..2587453	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU499_RS12725 (2587455)	2587455..2587934	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU499_RS12730 (2587909)	2587909..2588679	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208109 WP_000621175.1 NZ_CP077853:2585295-2585657 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208109 NZ_CP103570:2031043-2031146 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208109 WP_000948331.1 NZ_CP077853:2585128-2585298 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208109 NZ_CP103570:c2031184-2030913 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1