Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2563884..2564398 Replicon chromosome
Accession NZ_CP077853
Organism Staphylococcus aureus strain N5

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU499_RS12610 Protein ID WP_000581792.1
Coordinates 2564189..2564398 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU499_RS12605 Protein ID WP_000138932.1
Coordinates 2563884..2564177 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU499_RS12585 (2559147) 2559147..2559788 + 642 WP_000483153.1 thiamine phosphate synthase -
KU499_RS12590 (2559874) 2559874..2560746 + 873 WP_000725802.1 membrane protein insertase YidC -
KU499_RS12595 (2561559) 2561559..2562206 - 648 WP_001187630.1 HD domain-containing protein -
KU499_RS12600 (2562234) 2562234..2563718 - 1485 WP_000571549.1 cardiolipin synthase -
KU499_RS12605 (2563884) 2563884..2564177 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU499_RS12610 (2564189) 2564189..2564398 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU499_RS12615 (2564526) 2564526..2564663 + 138 WP_000828354.1 Lmo0850 family protein -
KU499_RS12620 (2564927) 2564927..2566129 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU499_RS12625 (2566447) 2566447..2567517 + 1071 WP_225798614.1 D-alanine--D-alanine ligase -
KU499_RS12630 (2567532) 2567532..2568890 + 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208108 WP_000581792.1 NZ_CP077853:2564189-2564398 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208108 NZ_CP103570:c1807200-1806823 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208108 WP_000138932.1 NZ_CP077853:2563884-2564177 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208108 NZ_CP103570:c1807658-1807290 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References