Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 207704..208218 Replicon chromosome
Accession NZ_CP077852
Organism Staphylococcus aureus strain N6

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU516_RS01165 Protein ID WP_000581792.1
Coordinates 207704..207913 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU516_RS01170 Protein ID WP_000138932.1
Coordinates 207925..208218 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU516_RS01145 (203212) 203212..204570 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KU516_RS01150 (204585) 204585..205655 - 1071 WP_225798614.1 D-alanine--D-alanine ligase -
KU516_RS01155 (205973) 205973..207175 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU516_RS01160 (207439) 207439..207576 - 138 WP_000828354.1 Lmo0850 family protein -
KU516_RS01165 (207704) 207704..207913 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU516_RS01170 (207925) 207925..208218 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU516_RS01175 (208384) 208384..209868 + 1485 WP_000571549.1 cardiolipin synthase -
KU516_RS01180 (209896) 209896..210543 + 648 WP_001187630.1 HD domain-containing protein -
KU516_RS01185 (211356) 211356..212228 - 873 WP_000725802.1 membrane protein insertase YidC -
KU516_RS01190 (212314) 212314..212955 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208085 WP_000581792.1 NZ_CP077852:c207913-207704 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208085 NZ_CP103563:c222032-221727 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208085 WP_000138932.1 NZ_CP077852:c208218-207925 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208085 NZ_CP103563:c222252-222034 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGTGGAAAATCAGGAAGGGATGGTTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References