Detailed information of TA system TA208076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2893025..2893652
• Replicon: chromosome
• Accession: NZ_CP077850
• Organism: Erwinia amylovora strain CP200242 isolate missinsg

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: KTR61_RS13010
• Protein ID: WP_004156337.1
• Coordinates: 2893434..2893652 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: KTR61_RS13005
• Protein ID: WP_013035913.1
• Coordinates: 2893025..2893414 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTR61_RS12985 (KTR61_12955)	2888044..2891190	+	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit AcrB	-
KTR61_RS12990 (KTR61_12960)	2891338..2891619	+	282	WP_004156350.1	type B 50S ribosomal protein L31	-
KTR61_RS12995 (KTR61_12965)	2891607..2891753	+	147	WP_004156349.1	type B 50S ribosomal protein L36	-
KTR61_RS13000 (KTR61_12970)	2892525..2892878	+	354	WP_004156342.1	hypothetical protein	-
KTR61_RS13005 (KTR61_12975)	2893025..2893414	+	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
KTR61_RS13010 (KTR61_12980)	2893434..2893652	+	219	WP_004156337.1	HHA domain-containing protein	Toxin
KTR61_RS13020 (KTR61_12990)	2893952..2894269	+	318	WP_004156334.1	MGMT family protein	-
KTR61_RS13025 (KTR61_12995)	2894292..2894849	-	558	WP_004156333.1	YbaY family lipoprotein	-
KTR61_RS13030 (KTR61_13000)	2895074..2895934	+	861	WP_004156324.1	acyl-CoA thioesterase II	-
KTR61_RS13035 (KTR61_13005)	2896009..2897310	-	1302	WP_004172214.1	ammonium transporter AmtB	-
KTR61_RS13040 (KTR61_13010)	2897328..2897666	-	339	WP_004156319.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T208076 WP_004156337.1 NZ_CP077850:2893434-2893652 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T208076 NZ_CP103562:c4204697-4204413 [Escherichia coli O25b:H4-ST131]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT208076 WP_013035913.1 NZ_CP077850:2893025-2893414 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT208076 NZ_CP103562:c4204933-4204700 [Escherichia coli O25b:H4-ST131]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A831EPQ0