Detailed information of TA system TA208074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2078261..2078873
• Replicon: chromosome
• Accession: NZ_CP077850
• Organism: Erwinia amylovora strain CP200242 isolate missinsg

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: KTR61_RS09205
• Protein ID: WP_012906750.1
• Coordinates: 2078694..2078873 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D4I356
• Locus tag: KTR61_RS09200
• Protein ID: WP_004157632.1
• Coordinates: 2078261..2078671 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTR61_RS09170 (KTR61_09160)	2073919..2074509	-	591	WP_223386180.1	DapH/DapD/GlmU-related protein	-
KTR61_RS09175 (KTR61_09165)	2075139..2075411	+	273	WP_004157637.1	hypothetical protein	-
KTR61_RS09180 (KTR61_09170)	2075422..2075595	+	174	WP_013036045.1	hypothetical protein	-
KTR61_RS09185 (KTR61_09175)	2075690..2075863	+	174	WP_004157636.1	hypothetical protein	-
KTR61_RS09190 (KTR61_09180)	2076121..2076333	+	213	WP_004157635.1	hypothetical protein	-
KTR61_RS09195 (KTR61_09185)	2077204..2078205	-	1002	WP_004157633.1	acyltransferase	-
KTR61_RS09200 (KTR61_09190)	2078261..2078671	-	411	WP_004157632.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KTR61_RS09205 (KTR61_09195)	2078694..2078873	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
KTR61_RS09210 (KTR61_09200)	2079393..2080121	-	729	WP_004157629.1	two-component system response regulator RstA	-
KTR61_RS09215 (KTR61_09205)	2080298..2080762	+	465	WP_004157626.1	hypothetical protein	-
KTR61_RS09220 (KTR61_09210)	2080766..2082157	-	1392	WP_004157625.1	amino acid permease	-
KTR61_RS09225 (KTR61_09215)	2082365..2083315	-	951	WP_004157624.1	DUF1471 family protein YdgH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2065495..2087719	22224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T208074 WP_012906750.1 NZ_CP077850:c2078873-2078694 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T208074 NZ_CP103562:3756762-3756980 [Escherichia coli O25b:H4-ST131]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14829.76 Da
• Isoelectric Point: 4.3623

>AT208074 WP_004157632.1 NZ_CP077850:c2078671-2078261 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT208074 NZ_CP103562:3756362-3756736 [Escherichia coli O25b:H4-ST131]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4

Antitoxin

• AlphaFold DB: A0A830ZVY0