Detailed information of TA system TA020807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 67295..67866
• Replicon: plasmid megaplasmid
• Accession: NC_004349
• Organism: Shewanella oneidensis MR-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q8E846
• Locus tag: SO_RS22545
• Protein ID: WP_011074407.1
• Coordinates: 67555..67866 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q8E847
• Locus tag: SO_RS22540
• Protein ID: WP_011074406.1
• Coordinates: 67295..67555 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SO_RS22505	62700..62918	-	219	Protein_74	IS3 family transposase	-
SO_RS22510	62973..63281	-	309	Protein_75	DDE-type integrase/transposase/recombinase	-
SO_RS22515	63339..64541	+	1203	WP_005054087.1	IS256-like element ISSod4 family transposase	-
SO_RS22520	64574..64960	-	387	Protein_77	IS6 family transposase	-
SO_RS22525	65042..65566	-	525	WP_164925948.1	hypothetical protein	-
SO_RS22530	65893..66451	-	559	Protein_79	IS6 family transposase	-
SO_RS22535	66535..67125	-	591	WP_011074405.1	recombinase family protein	-
SO_RS22540	67295..67555	+	261	WP_011074406.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SO_RS22545	67555..67866	+	312	WP_011074407.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SO_RS22550	67890..69248	+	1359	Protein_83	DUF4158 domain-containing protein	-
SO_RS22555	69314..70473	+	1160	WP_141135407.1	IS3-like element ISSod1 family transposase	-
SO_RS22560	70482..72206	+	1725	Protein_85	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..161613	161613
	-	inside	IScluster/Tn	-	-	45061..76483	31422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-61)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11796.59 Da
• Isoelectric Point: 8.5262

>T20807 WP_011074407.1 NC_004349:67555-67866 [Shewanella oneidensis MR-1]
MAVTYHLTPDAQSDLIGIHRFTLAQWGATQSKTYLSGLKQTIQLLAETPTLGKNRPEVRMNVFSFPYSSHVIYYIQHEHQ
FVVFGILHKSMVPLTHLAERETI

Nucleotide

• Length: 312 bp

>T20807 NC_004349:67555-67866 [Shewanella oneidensis MR-1]
ATGGCAGTAACTTATCATTTAACACCTGATGCACAGTCTGATCTGATAGGCATTCACCGCTTTACGTTAGCCCAATGGGG
GGCTACTCAGTCAAAGACCTATTTATCCGGACTTAAACAAACGATTCAGCTGCTGGCTGAAACACCCACCCTTGGGAAAA
ATAGACCCGAGGTACGCATGAATGTGTTTAGTTTTCCTTATTCAAGTCACGTCATCTATTACATCCAACATGAGCATCAA
TTCGTTGTATTTGGGATCTTACATAAAAGTATGGTTCCACTTACTCATCTCGCAGAGCGGGAAACCATCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9329.65 Da
• Isoelectric Point: 4.9373

>AT20807 WP_011074406.1 NC_004349:67295-67555 [Shewanella oneidensis MR-1]
MNTLSANEAKIHFGDLLLKAQQSPIQINKNGKPVAVVISADEYQSIEALKLHLLQSKAVKAMTDIQTGNLVDGNTFFDEL
AAGLYD

Nucleotide

• Length: 261 bp

>AT20807 NC_004349:67295-67555 [Shewanella oneidensis MR-1]
ATGAACACTCTTTCAGCCAATGAAGCAAAAATTCACTTTGGAGATTTGTTGCTCAAAGCCCAACAATCTCCAATCCAAAT
TAATAAAAATGGCAAGCCTGTAGCCGTTGTAATTTCTGCTGATGAATACCAAAGTATCGAGGCGCTAAAATTACACTTGC
TACAATCTAAAGCGGTGAAAGCCATGACCGACATCCAAACGGGCAATTTGGTTGACGGTAATACCTTTTTTGATGAGCTG
GCTGCAGGGCTGTATGACTAA

Structures

Toxin

• AlphaFold DB: Q8E846

Antitoxin

• AlphaFold DB: Q8E847