208054

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2762..3350 Replicon plasmid pDETAB10
Accession NZ_CP077834
Organism Acinetobacter baumannii strain DETAB-P43

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag KU224_RS19680 Protein ID WP_000438826.1
Coordinates 2762..3049 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag KU224_RS19685 Protein ID WP_001983304.1
Coordinates 3036..3350 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU224_RS19660 (KU224_19590) 1..951 + 951 WP_001205343.1 replication initiation protein RepM -
KU224_RS19665 (KU224_19595) 944..1519 + 576 WP_001096616.1 plasmid replication DNA-binding protein -
KU224_RS19670 (KU224_19600) 1539..1682 + 144 WP_001125246.1 hypothetical protein -
KU224_RS19675 (KU224_19605) 1871..2389 - 519 WP_000447193.1 tetratricopeptide repeat protein -
KU224_RS19680 (KU224_19610) 2762..3049 + 288 WP_000438826.1 BrnT family toxin Toxin
KU224_RS19685 (KU224_19615) 3036..3350 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
KU224_RS19690 (KU224_19620) 3478..5889 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
KU224_RS19695 (KU224_19625) 6194..6655 + 462 WP_000761346.1 DIP1984 family protein -
KU224_RS19700 (KU224_19630) 6978..7151 - 174 WP_001282484.1 hypothetical protein -
KU224_RS19705 (KU224_19635) 7151..7522 - 372 WP_000504218.1 hypothetical protein -
KU224_RS19710 (KU224_19640) 7794..8048 - 255 WP_001014303.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..8731 8731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T208054 WP_000438826.1 NZ_CP077834:2762-3049 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T208054 NZ_CP103560:c4748775-4748434 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT208054 WP_001983304.1 NZ_CP077834:3036-3350 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT208054 NZ_CP103560:c4749137-4748796 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References