Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1250819..1251438 Replicon chromosome
Accession NZ_CP077824
Organism Klebsiella pneumoniae strain FF1009

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU661_RS05910 Protein ID WP_002892050.1
Coordinates 1250819..1251037 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU661_RS05915 Protein ID WP_002892066.1
Coordinates 1251064..1251438 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU661_RS05875 (KU661_05865) 1246866..1247129 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KU661_RS05880 (KU661_05870) 1247129..1247269 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU661_RS05885 (KU661_05875) 1247266..1247964 - 699 WP_117045594.1 GNAT family protein -
KU661_RS05890 (KU661_05880) 1248065..1249516 + 1452 WP_117045592.1 PLP-dependent aminotransferase family protein -
KU661_RS05895 (KU661_05885) 1249491..1249961 - 471 WP_002892026.1 YlaC family protein -
KU661_RS05900 (KU661_05890) 1249982..1250122 + 141 WP_004147370.1 hypothetical protein -
KU661_RS05905 (KU661_05895) 1250094..1250660 - 567 WP_002892030.1 maltose O-acetyltransferase -
KU661_RS05910 (KU661_05900) 1250819..1251037 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KU661_RS05915 (KU661_05905) 1251064..1251438 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU661_RS05920 (KU661_05910) 1251924..1255070 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU661_RS05925 (KU661_05915) 1255093..1256286 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T208030 WP_002892050.1 NZ_CP077824:c1251037-1250819 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T208030 NZ_CP103559:c2234782-2234675 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT208030 WP_002892066.1 NZ_CP077824:c1251438-1251064 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT208030 NZ_CP103559:2234829-2234895 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References