Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 628604..629414 Replicon chromosome
Accession NZ_CP077824
Organism Klebsiella pneumoniae strain FF1009

Toxin (Protein)


Gene name KacT Uniprot ID A0A6G4MHE5
Locus tag KU661_RS02935 Protein ID WP_065801222.1
Coordinates 628881..629414 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KU661_RS02930 Protein ID WP_002887278.1
Coordinates 628604..628870 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU661_RS02910 (KU661_02905) 624476..624820 - 345 WP_021441118.1 cation efflux system protein CusF -
KU661_RS02915 (KU661_02910) 624838..626223 - 1386 WP_009484676.1 efflux transporter outer membrane subunit -
KU661_RS02920 (KU661_02915) 626395..627078 + 684 WP_004214143.1 copper response regulator transcription factor CusR -
KU661_RS02925 (KU661_02920) 627068..628501 + 1434 WP_065801221.1 Cu(+)/Ag(+) sensor histidine kinase -
KU661_RS02930 (KU661_02925) 628604..628870 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KU661_RS02935 (KU661_02930) 628881..629414 + 534 WP_065801222.1 type II toxin-antitoxin system toxin KacT Toxin
KU661_RS02940 (KU661_02935) 629462..630583 - 1122 WP_004214138.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19868.73 Da        Isoelectric Point: 5.2614

>T208029 WP_065801222.1 NZ_CP077824:628881-629414 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRSYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKVFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T208029 NZ_CP103559:c2234247-2234140 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT208029 WP_002887278.1 NZ_CP077824:628604-628870 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT208029 NZ_CP103559:2234295-2234361 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4MHE5

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References