Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 27254..27879 Replicon chromosome
Accession NZ_CP077824
Organism Klebsiella pneumoniae strain FF1009

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KU661_RS00130 Protein ID WP_002882817.1
Coordinates 27496..27879 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KU661_RS00125 Protein ID WP_004150355.1
Coordinates 27254..27496 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU661_RS00100 (KU661_00100) 23245..23844 + 600 WP_004151865.1 glucose-1-phosphatase -
KU661_RS00105 (KU661_00105) 23838..24698 + 861 WP_004146232.1 virulence factor BrkB family protein -
KU661_RS00110 (KU661_00110) 24695..25132 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KU661_RS00115 (KU661_00115) 25177..26118 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KU661_RS00120 (KU661_00120) 26132..27049 - 918 WP_110230358.1 alpha/beta hydrolase -
KU661_RS00125 (KU661_00125) 27254..27496 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KU661_RS00130 (KU661_00130) 27496..27879 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KU661_RS00135 (KU661_00135) 28053..28982 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KU661_RS00140 (KU661_00140) 28979..29614 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KU661_RS00145 (KU661_00145) 29611..30513 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T208026 WP_002882817.1 NZ_CP077824:27496-27879 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T208026 NZ_CP103559:1893519-1893770 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT208026 WP_004150355.1 NZ_CP077824:27254-27496 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT208026 NZ_CP103559:1893767-1894021 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References