Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3957747..3958366 Replicon chromosome
Accession NZ_CP077823
Organism Klebsiella africana strain FF1019

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU660_RS19025 Protein ID WP_002892050.1
Coordinates 3958148..3958366 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A223UF87
Locus tag KU660_RS19020 Protein ID WP_025710692.1
Coordinates 3957747..3958121 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU660_RS19010 (3952902) 3952902..3954095 + 1194 WP_136033418.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU660_RS19015 (3954118) 3954118..3957264 + 3147 WP_203033068.1 multidrug efflux RND transporter permease subunit AcrB -
KU660_RS19020 (3957747) 3957747..3958121 + 375 WP_025710692.1 Hha toxicity modulator TomB Antitoxin
KU660_RS19025 (3958148) 3958148..3958366 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU660_RS19030 (3958523) 3958523..3959089 + 567 WP_203033069.1 maltose O-acetyltransferase -
KU660_RS19035 (3959082) 3959082..3959189 - 108 Protein_3736 hypothetical protein -
KU660_RS19040 (3959226) 3959226..3959696 + 471 WP_025710690.1 YlaC family protein -
KU660_RS19045 (3959671) 3959671..3961122 - 1452 WP_203033070.1 PLP-dependent aminotransferase family protein -
KU660_RS19050 (3961223) 3961223..3961921 + 699 WP_136033425.1 GNAT family protein -
KU660_RS19055 (3961918) 3961918..3962058 - 141 WP_136033621.1 type B 50S ribosomal protein L36 -
KU660_RS19060 (3962058) 3962058..3962321 - 264 WP_080924893.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T208022 WP_002892050.1 NZ_CP077823:3958148-3958366 [Klebsiella africana]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T208022 NZ_CP103559:c829925-829548 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14398.04 Da        Isoelectric Point: 4.7295

>AT208022 WP_025710692.1 NZ_CP077823:3957747-3958121 [Klebsiella africana]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIDQVDEYL
DDTFTLFSNYGINSSDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT208022 NZ_CP103559:c830383-830015 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A223UF87

References