Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1760895..1761485 Replicon chromosome
Accession NZ_CP077823
Organism Klebsiella africana strain FF1019

Toxin (Protein)


Gene name pemK Uniprot ID A0A8B6IZR4
Locus tag KU660_RS08430 Protein ID WP_040245064.1
Coordinates 1761153..1761485 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag KU660_RS08425 Protein ID WP_000288812.1
Coordinates 1760895..1761152 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU660_RS08400 (1756713) 1756713..1756799 + 87 Protein_1645 type II toxin-antitoxin system PemK/MazF family toxin -
KU660_RS08405 (1757150) 1757150..1758898 + 1749 WP_251354982.1 hypothetical protein -
KU660_RS08410 (1758977) 1758977..1759534 + 558 Protein_1647 hypothetical protein -
KU660_RS08415 (1759893) 1759893..1760357 + 465 WP_251354983.1 hypothetical protein -
KU660_RS08420 (1760354) 1760354..1760560 + 207 WP_251354984.1 helix-turn-helix transcriptional regulator -
KU660_RS08425 (1760895) 1760895..1761152 + 258 WP_000288812.1 antitoxin Antitoxin
KU660_RS08430 (1761153) 1761153..1761485 + 333 WP_040245064.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KU660_RS08440 (1761807) 1761807..1763243 + 1437 WP_251354986.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KU660_RS08450 (1763569) 1763569..1763718 - 150 Protein_1653 antitoxin -
KU660_RS08455 (1763921) 1763921..1764223 - 303 WP_061153979.1 XRE family transcriptional regulator -
KU660_RS08460 (1764228) 1764228..1764581 - 354 WP_008804168.1 type II toxin-antitoxin system RelE/ParE family toxin -
KU660_RS08465 (1764994) 1764994..1765452 + 459 WP_136032098.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11885.75 Da        Isoelectric Point: 10.1839

>T208010 WP_040245064.1 NZ_CP077823:1761153-1761485 [Klebsiella africana]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAMLS

Download         Length: 333 bp

>T208010 NZ_CP103557:c4594883-4594533 [Escherichia coli O25b:H4-ST131]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT208010 WP_000288812.1 NZ_CP077823:1760895-1761152 [Klebsiella africana]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT208010 NZ_CP103557:c4595128-4594877 [Escherichia coli O25b:H4-ST131]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6IZR4

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References