Detailed information of TA system TA020801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 663421..664187
• Replicon: chromosome
• Accession: NC_004347
• Organism: Shewanella oneidensis MR-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q8EJ37
• Locus tag: SO_RS03010
• Protein ID: WP_011070962.1
• Coordinates: 663421..663915 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q8EJ36
• Locus tag: SO_RS03015
• Protein ID: WP_011070963.1
• Coordinates: 663912..664187 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SO_RS03000 (SO_0633)	660661..662967	+	2307	WP_011070960.1	penicillin-binding protein 1B	-
SO_RS03005 (SO_0635)	663045..663323	+	279	WP_011070961.1	peptidylprolyl isomerase	-
SO_RS03010 (SO_0636)	663421..663915	-	495	WP_011070962.1	GNAT family N-acetyltransferase	Toxin
SO_RS03015 (SO_0637)	663912..664187	-	276	WP_011070963.1	DUF1778 domain-containing protein	Antitoxin
SO_RS03020 (SO_0639)	664604..667717	-	3114	WP_011070964.1	M9 family metallopeptidase	-
SO_RS03025 (SO_0640)	668166..669164	+	999	WP_011070965.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-155)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18408.04 Da
• Isoelectric Point: 8.0487

>T20801 WP_011070962.1 NC_004347:c663915-663421 [Shewanella oneidensis MR-1]
MNTVLLDKDKHDRNRFNCGIEALNNYIKVMASQQAKKDNTRTFVLEDDNNNAHIIGFYTLTMTPIDLKALPDKLQKKHQS
STSGGLIARLAVDERYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYEPYGFQAFQETENKLFITIADIR
ASLG

Nucleotide

• Length: 495 bp

>T20801 NC_004347:c663915-663421 [Shewanella oneidensis MR-1]
ATGAATACGGTACTTCTCGACAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGTATTGAAGCTCTCAACAATTACAT
AAAAGTCATGGCGAGTCAGCAAGCCAAGAAAGACAATACCAGAACGTTCGTTTTAGAAGATGATAACAACAACGCCCATA
TCATCGGCTTTTACACACTGACGATGACACCCATTGATTTGAAGGCACTGCCGGACAAGTTGCAGAAGAAGCACCAATCT
TCAACATCAGGAGGGCTCATTGCTCGTTTAGCTGTCGATGAGCGATACAAAGGGAAAGGTTTTGGCGAGTGGCTGCTTAT
TGATGCCCTCCGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTTCCTGTTGTCATCGTTGATGCCAAGGACGGAGCCA
AACACTTCTATGAGCCCTATGGATTTCAAGCGTTTCAAGAGACCGAAAACAAGCTCTTCATCACCATAGCAGATATCAGA
GCAAGCTTGGGCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9857.37 Da
• Isoelectric Point: 6.2988

>AT20801 WP_011070963.1 NC_004347:c664187-663912 [Shewanella oneidensis MR-1]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQIIEREQALKLSQADAVLLMEALDNPAAVNTKLKL
ASERYESKTQQ

Nucleotide

• Length: 276 bp

>AT20801 NC_004347:c664187-663912 [Shewanella oneidensis MR-1]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCAGCTCTCGC
CGGCATGTCTAGTATTAACTCATTTGTTTTAAATGCGGCAATCGAAAAAGCAAAACAAATCATTGAACGAGAGCAAGCTC
TAAAACTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTCGATAATCCAGCGGCAGTCAATACAAAACTCAAATTG
GCGTCGGAACGTTATGAGAGCAAAACCCAGCAATGA

Structures

Toxin

• AlphaFold DB: Q8EJ37

Antitoxin

• AlphaFold DB: Q8EJ36