Detailed information of TA system TA208008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 738493..739268
• Replicon: chromosome
• Accession: NZ_CP077823
• Organism: Klebsiella africana strain FF1019

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: KU660_RS03635
• Protein ID: WP_251354791.1
• Coordinates: 738783..739268 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7U2ZSW0
• Locus tag: KU660_RS03630
• Protein ID: WP_025710477.1
• Coordinates: 738493..738786 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU660_RS03610 (733699)	733699..734301	-	603	WP_251354788.1	short chain dehydrogenase	-
KU660_RS03615 (734399)	734399..735310	+	912	WP_251354789.1	LysR family transcriptional regulator	-
KU660_RS03620 (735311)	735311..736459	-	1149	WP_136031430.1	PLP-dependent aspartate aminotransferase family protein	-
KU660_RS03625 (736470)	736470..737846	-	1377	WP_251354790.1	pyridoxal-phosphate dependent enzyme	-
KU660_RS03630 (738493)	738493..738786	+	294	WP_025710477.1	DUF1778 domain-containing protein	Antitoxin
KU660_RS03635 (738783)	738783..739268	+	486	WP_251354791.1	GNAT family N-acetyltransferase	Toxin
KU660_RS03640 (739974)	739974..740568	+	595	Protein_715	hypothetical protein	-
KU660_RS03645 (740665)	740665..740881	+	217	Protein_716	transposase	-
KU660_RS03655 (741559)	741559..742272	-	714	WP_203033620.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	740665..740817	152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17466.38 Da
• Isoelectric Point: 8.2962

>T208008 WP_251354791.1 NZ_CP077823:738783-739268 [Klebsiella africana]
MISAPEPLHAGHILTPFCSGVDSMDNWLKQRAMKNQLSGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLGDLVH
A

Nucleotide

• Length: 486 bp

>T208008 NZ_CP103557:c4415647-4415360 [Escherichia coli O25b:H4-ST131]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10990.61 Da
• Isoelectric Point: 8.6141

>AT208008 WP_025710477.1 NZ_CP077823:738493-738786 [Klebsiella africana]
MPAANSMTMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT208008 NZ_CP103557:c4415373-4415071 [Escherichia coli O25b:H4-ST131]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U2ZSW0