Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5070035..5070660 Replicon chromosome
Accession NZ_CP077820
Organism Klebsiella pneumoniae strain FF1013

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KU668_RS24690 Protein ID WP_002882817.1
Coordinates 5070277..5070660 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KU668_RS24685 Protein ID WP_004150355.1
Coordinates 5070035..5070277 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU668_RS24660 (5066026) 5066026..5066625 + 600 WP_004151865.1 glucose-1-phosphatase -
KU668_RS24665 (5066619) 5066619..5067479 + 861 WP_004146232.1 virulence factor BrkB family protein -
KU668_RS24670 (5067476) 5067476..5067913 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KU668_RS24675 (5067958) 5067958..5068899 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KU668_RS24680 (5068913) 5068913..5069830 - 918 WP_065803683.1 alpha/beta hydrolase -
KU668_RS24685 (5070035) 5070035..5070277 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KU668_RS24690 (5070277) 5070277..5070660 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KU668_RS24695 (5070834) 5070834..5071763 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KU668_RS24700 (5071760) 5071760..5072395 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KU668_RS24705 (5072392) 5072392..5073294 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T208006 WP_002882817.1 NZ_CP077820:5070277-5070660 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T208006 NZ_CP103557:c4037634-4036714 [Escherichia coli O25b:H4-ST131]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT208006 WP_004150355.1 NZ_CP077820:5070035-5070277 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT208006 NZ_CP103557:c4038403-4037621 [Escherichia coli O25b:H4-ST131]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References