Detailed information of TA system TA020799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1774316..1774887
• Replicon: chromosome
• Accession: NC_004342
• Organism: Leptospira interrogans serovar Lai str. 56601

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q8F5A3
• Locus tag: LA_RS07350
• Protein ID: WP_000240418.1
• Coordinates: 1774546..1774887 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: LA_RS07345
• Protein ID: WP_000443825.1
• Coordinates: 1774316..1774552 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA_RS07315 (LA1775)	1770839..1771315	+	477	WP_000691822.1	hypothetical protein	-
LA_RS07320 (LA1776)	1771564..1771767	-	204	WP_000532164.1	hypothetical protein	-
LA_RS07325	1771827..1772201	-	375	WP_001206461.1	hypothetical protein	-
LA_RS07330	1772206..1772471	-	266	Protein_1452	BrnT family toxin	-
LA_RS07335 (LA1778)	1772583..1772876	+	294	WP_100220714.1	hypothetical protein	-
LA_RS07340 (LA1779)	1773189..1773980	+	792	WP_000703027.1	hypothetical protein	-
LA_RS07345 (LA1780)	1774316..1774552	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LA_RS07350 (LA1781)	1774546..1774887	+	342	WP_000240418.1	endoribonuclease MazF	Toxin
LA_RS07355 (LA1782)	1774955..1775337	-	383	Protein_1457	hypothetical protein	-
LA_RS07360 (LA1783)	1775337..1775603	-	267	WP_000844450.1	hypothetical protein	-
LA_RS07365	1775664..1775992	-	329	Protein_1459	hypothetical protein	-
LA_RS07370 (LA1786)	1775995..1776717	-	723	WP_000414906.1	hypothetical protein	-
LA_RS07375	1776930..1777136	+	207	WP_080010645.1	BrnT family toxin	-
LA_RS07380 (LA1789)	1777865..1778326	+	462	WP_000811796.1	hypothetical protein	-
LA_RS19530 (LA1791)	1778689..1779042	+	354	WP_002095367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12918.12 Da
• Isoelectric Point: 9.9595

>T20799 WP_000240418.1 NC_004342:1774546-1774887 [Leptospira interrogans serovar Lai str. 56601]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Nucleotide

• Length: 342 bp

>T20799 NC_004342:1774546-1774887 [Leptospira interrogans serovar Lai str. 56601]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGTTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT20799 WP_000443825.1 NC_004342:1774316-1774552 [Leptospira interrogans serovar Lai str. 56601]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT20799 NC_004342:1774316-1774552 [Leptospira interrogans serovar Lai str. 56601]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A4D8SL30

Antitoxin

• AlphaFold DB: A0A0F6HC33