Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3065109..3065728 Replicon chromosome
Accession NZ_CP077818
Organism Klebsiella pneumoniae strain FF1023

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU670_RS14705 Protein ID WP_002892050.1
Coordinates 3065109..3065327 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU670_RS14710 Protein ID WP_002892066.1
Coordinates 3065354..3065728 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU670_RS14670 (KU670_14670) 3061156..3061419 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KU670_RS14675 (KU670_14675) 3061419..3061559 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU670_RS14680 (KU670_14680) 3061556..3062254 - 699 WP_004178762.1 GNAT family protein -
KU670_RS14685 (KU670_14685) 3062355..3063806 + 1452 WP_087831258.1 PLP-dependent aminotransferase family protein -
KU670_RS14690 (KU670_14690) 3063781..3064251 - 471 WP_002892026.1 YlaC family protein -
KU670_RS14695 (KU670_14695) 3064272..3064412 + 141 WP_004147370.1 hypothetical protein -
KU670_RS14700 (KU670_14700) 3064384..3064950 - 567 WP_002892030.1 maltose O-acetyltransferase -
KU670_RS14705 (KU670_14705) 3065109..3065327 - 219 WP_002892050.1 HHA domain-containing protein Toxin
KU670_RS14710 (KU670_14710) 3065354..3065728 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU670_RS14715 (KU670_14715) 3066214..3069360 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU670_RS14720 (KU670_14720) 3069383..3070576 - 1194 WP_251347287.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207979 WP_002892050.1 NZ_CP077818:c3065327-3065109 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207979 NZ_CP103554:c57556-57248 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207979 WP_002892066.1 NZ_CP077818:c3065728-3065354 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207979 NZ_CP103554:c57795-57559 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References