Detailed information of TA system TA207966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3504354..3505164
• Replicon: chromosome
• Accession: NZ_CP077816
• Organism: Klebsiella pneumoniae strain FF1043

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KU669_RS17045
• Protein ID: WP_152210901.1
• Coordinates: 3504354..3504887 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: KU669_RS17050
• Protein ID: WP_002887278.1
• Coordinates: 3504898..3505164 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU669_RS17040 (KU669_17005)	3503185..3504306	+	1122	WP_004186850.1	cupin domain-containing protein	-
KU669_RS17045 (KU669_17010)	3504354..3504887	-	534	WP_152210901.1	type II toxin-antitoxin system toxin KacT	Toxin
KU669_RS17050 (KU669_17015)	3504898..3505164	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
KU669_RS17055 (KU669_17020)	3505267..3506700	-	1434	WP_004186848.1	Cu(+)/Ag(+) sensor histidine kinase	-
KU669_RS17060 (KU669_17025)	3506690..3507373	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
KU669_RS17065 (KU669_17030)	3507546..3508931	+	1386	WP_064152126.1	efflux transporter outer membrane subunit	-
KU669_RS17070 (KU669_17035)	3508949..3509293	+	345	WP_004894569.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (93-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19838.71 Da
• Isoelectric Point: 5.7068

>T207966 WP_152210901.1 NZ_CP077816:c3504887-3504354 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYRNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T207966 NZ_CP103550:799924-800334 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT207966 WP_002887278.1 NZ_CP077816:c3505164-3504898 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT207966 NZ_CP103550:799677-799943 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1