Detailed information of TA system TA207962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 756061..756617
• Replicon: chromosome
• Accession: NZ_CP077816
• Organism: Klebsiella pneumoniae strain FF1043

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU669_RS03635
• Protein ID: WP_251357141.1
• Coordinates: 756309..756617 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KU669_RS03630
• Protein ID: WP_251357137.1
• Coordinates: 756061..756291 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU669_RS03600 (KU669_03595)	751072..751929	+	858	WP_251357126.1	hypothetical protein	-
KU669_RS03605 (KU669_03600)	751931..752539	+	609	WP_251357127.1	hypothetical protein	-
KU669_RS03610 (KU669_03605)	752536..752925	+	390	WP_251357128.1	hypothetical protein	-
KU669_RS03615 (KU669_03610)	752959..753342	+	384	WP_251357129.1	zinc ribbon domain-containing protein	-
KU669_RS03620 (KU669_03615)	753712..754479	+	768	WP_251357130.1	hypothetical protein	-
KU669_RS03625 (KU669_03620)	754937..755911	+	975	WP_251357131.1	P63C domain-containing protein	-
KU669_RS03630 (KU669_03625)	756061..756291	+	231	WP_251357137.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KU669_RS03635 (KU669_03630)	756309..756617	+	309	WP_251357141.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU669_RS03640 (KU669_03635)	756682..757041	+	360	WP_123836709.1	hypothetical protein	-
KU669_RS03645 (KU669_03640)	757049..758281	+	1233	WP_251357145.1	baseplate J/gp47 family protein	-
KU669_RS03650 (KU669_03645)	758281..758832	+	552	WP_251357147.1	hypothetical protein	-
KU669_RS03655 (KU669_03650)	758814..759701	+	888	WP_251357149.1	hypothetical protein	-
KU669_RS03660 (KU669_03655)	759685..760296	+	612	WP_251357151.1	methyltransferase domain-containing protein	-
KU669_RS03665 (KU669_03660)	760299..760898	+	600	WP_072045291.1	DUF2612 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	697829..779441	81612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-100)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11168.89 Da
• Isoelectric Point: 9.9255

>T207962 WP_251357141.1 NZ_CP077816:756309-756617 [Klebsiella pneumoniae]
VVLIDFTPSAGSEQGGKRPALVVSETYFNQHDRFVVLCPITSKAKGYGFEVPIRGTQKTTGVILSNQFKSLDYRARNMVV
VDSVDAKTLAAVQHNIKQILGM

Nucleotide

• Length: 309 bp

>T207962 NZ_CP103548:4650570-4651103 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8101.35 Da
• Isoelectric Point: 4.4368

>AT207962 WP_251357137.1 NZ_CP077816:756061-756291 [Klebsiella pneumoniae]
MATVVAKWGNAVGVRIPAALLDQAGITVGDQVEFCVKGGMLGLKKTGPTLQELMSKCTVENSHDEYFSESMGEELL

Nucleotide

• Length: 231 bp

>AT207962 NZ_CP103548:4650293-4650559 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA