Detailed information of TA system TA207953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 121099..121903
• Replicon: plasmid pFF1076_1
• Accession: NZ_CP077815
• Organism: Klebsiella pneumoniae strain FF1076

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KU667_RS26555
• Protein ID: WP_072198182.1
• Coordinates: 121099..121626 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: KU667_RS26560
• Protein ID: WP_048264996.1
• Coordinates: 121634..121903 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU667_RS26530 (KU667_26435)	116739..117920	+	1182	WP_048977967.1	MFS transporter	-
KU667_RS26535 (KU667_26440)	118039..118647	-	609	WP_049095464.1	short chain dehydrogenase	-
KU667_RS26540 (KU667_26445)	118745..119647	+	903	WP_251341151.1	LysR family transcriptional regulator	-
KU667_RS26550 (KU667_26455)	120707..121015	-	309	WP_048291890.1	hypothetical protein	-
KU667_RS26555 (KU667_26460)	121099..121626	-	528	WP_072198182.1	GNAT family N-acetyltransferase	Toxin
KU667_RS26560 (KU667_26465)	121634..121903	-	270	WP_048264996.1	DUF1778 domain-containing protein	Antitoxin
KU667_RS26565 (KU667_26470)	122537..124900	-	2364	WP_048264995.1	hypothetical protein	-
KU667_RS26570 (KU667_26475)	124915..125661	-	747	WP_048978068.1	ABC transporter permease	-
KU667_RS26575 (KU667_26480)	125658..126650	-	993	WP_060598672.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174986	174986
	-	flank	IS/Tn	-	-	119959..120462	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (54-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 20078.04 Da
• Isoelectric Point: 5.7389

>T207953 WP_072198182.1 NZ_CP077815:c121626-121099 [Klebsiella pneumoniae]
VMQELAIEMINDEFDYDIGEFDCGEETLNTFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Nucleotide

• Length: 528 bp

>T207953 NZ_CP103548:c1528557-1528240 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10148.65 Da
• Isoelectric Point: 10.1733

>AT207953 WP_048264996.1 NZ_CP077815:c121903-121634 [Klebsiella pneumoniae]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Nucleotide

• Length: 270 bp

>AT207953 NZ_CP103548:c1528164-1527868 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA