Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 100315..100840 Replicon plasmid pFF1076_1
Accession NZ_CP077815
Organism Klebsiella pneumoniae strain FF1076

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag KU667_RS26445 Protein ID WP_004197633.1
Coordinates 100315..100620 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag KU667_RS26450 Protein ID WP_015632547.1
Coordinates 100622..100840 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU667_RS26420 (KU667_26325) 95893..97040 + 1148 WP_251341147.1 IS3 family transposase -
KU667_RS26425 (KU667_26330) 97003..97380 + 378 WP_158672279.1 DUF1173 family protein -
KU667_RS26430 (KU667_26335) 97529..97834 + 306 WP_126034233.1 hypothetical protein -
KU667_RS26435 (KU667_26340) 98156..98941 - 786 WP_048291886.1 site-specific integrase -
KU667_RS26440 (KU667_26345) 99140..100128 - 989 Protein_104 hypothetical protein -
KU667_RS26445 (KU667_26350) 100315..100620 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
KU667_RS26450 (KU667_26355) 100622..100840 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KU667_RS26455 (KU667_26360) 101119..102492 + 1374 WP_251341148.1 hypothetical protein -
KU667_RS26460 (KU667_26365) 102834..103262 - 429 WP_023345594.1 DUF4440 domain-containing protein -
KU667_RS26465 (KU667_26370) 103375..104220 - 846 WP_023345593.1 SDR family oxidoreductase -
KU667_RS26470 (KU667_26375) 104371..104718 + 348 WP_032638146.1 helix-turn-helix domain-containing protein -
KU667_RS26475 (KU667_26380) 104830..105414 + 585 WP_251341149.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..174986 174986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T207951 WP_004197633.1 NZ_CP077815:c100620-100315 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T207951 NZ_CP103548:53928-54410 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT207951 WP_015632547.1 NZ_CP077815:c100840-100622 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT207951 NZ_CP103548:53674-53940 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References