Detailed information of TA system TA207950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 50722..51632
• Replicon: plasmid pFF1076_1
• Accession: NZ_CP077815
• Organism: Klebsiella pneumoniae strain FF1076

Toxin (Protein)

• Gene name: -
• Uniprot ID: W9B9V2
• Locus tag: KU667_RS26165
• Protein ID: WP_016528981.1
• Coordinates: 50722..51192 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: KU667_RS26170
• Protein ID: WP_004026357.1
• Coordinates: 51189..51632 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU667_RS26130 (KU667_26045)	45893..46378	-	486	WP_021312536.1	GNAT family N-acetyltransferase	-
KU667_RS26135 (KU667_26050)	46366..46632	-	267	WP_003026799.1	DUF1778 domain-containing protein	-
KU667_RS26140 (KU667_26055)	46765..47193	-	429	WP_004901287.1	GFA family protein	-
KU667_RS26150	49475..49567	-	93	WP_223175079.1	DUF5431 family protein	-
KU667_RS26155	49534..49629	-	96	Protein_47	Hok/Gef family protein	-
KU667_RS26160 (KU667_26070)	50352..50612	+	261	WP_251341182.1	hypothetical protein	-
KU667_RS26165 (KU667_26075)	50722..51192	-	471	WP_016528981.1	RES family NAD+ phosphorylase	Toxin
KU667_RS26170 (KU667_26080)	51189..51632	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
KU667_RS26175 (KU667_26085)	51733..52245	-	513	Protein_51	DUF4113 domain-containing protein	-
KU667_RS26180 (KU667_26090)	52347..52568	-	222	WP_072060588.1	DUF2188 domain-containing protein	-
KU667_RS26185 (KU667_26095)	53019..53729	+	711	WP_048291870.1	lysozyme inhibitor LprI family protein	-
KU667_RS26190 (KU667_26100)	53729..54070	+	342	WP_196095282.1	hypothetical protein	-
KU667_RS26195 (KU667_26105)	54495..55564	+	1070	WP_251341192.1	IS3 family transposase	-
KU667_RS26200 (KU667_26110)	55775..56311	+	537	WP_032414401.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174986	174986
	-	flank	IS/Tn	-	-	47394..48119	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17603.85 Da
• Isoelectric Point: 4.4093

>T207950 WP_016528981.1 NZ_CP077815:c51192-50722 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTWSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T207950 NZ_CP103546:c94370-94065 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT207950 WP_004026357.1 NZ_CP077815:c51632-51189 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT207950 NZ_CP103546:c94590-94372 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: W9B9V2

Antitoxin

• AlphaFold DB: A0A6P1V3Q9