Detailed information of TA system TA207949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 45893..46632
• Replicon: plasmid pFF1076_1
• Accession: NZ_CP077815
• Organism: Klebsiella pneumoniae strain FF1076

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KU667_RS26130
• Protein ID: WP_021312536.1
• Coordinates: 45893..46378 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KU667_RS26135
• Protein ID: WP_003026799.1
• Coordinates: 46366..46632 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU667_RS26095 (KU667_26015)	41152..41472	-	321	WP_251341179.1	hypothetical protein	-
KU667_RS26100 (KU667_26020)	41568..42635	-	1068	WP_251341180.1	hypothetical protein	-
KU667_RS26105 (KU667_26025)	42704..43000	-	297	WP_048291874.1	hypothetical protein	-
KU667_RS26110 (KU667_26030)	43234..43611	+	378	WP_032719541.1	hypothetical protein	-
KU667_RS26115 (KU667_26035)	43808..44782	-	975	WP_251341181.1	Hok/Gef family protein	-
KU667_RS26120 (KU667_26040)	45388..45510	-	123	WP_085842394.1	Hok/Gef family protein	-
KU667_RS26125	45455..45607	-	153	Protein_41	DUF5431 family protein	-
KU667_RS26130 (KU667_26045)	45893..46378	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
KU667_RS26135 (KU667_26050)	46366..46632	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KU667_RS26140 (KU667_26055)	46765..47193	-	429	WP_004901287.1	GFA family protein	-
KU667_RS26150	49475..49567	-	93	WP_223175079.1	DUF5431 family protein	-
KU667_RS26155	49534..49629	-	96	Protein_47	Hok/Gef family protein	-
KU667_RS26160 (KU667_26070)	50352..50612	+	261	WP_251341182.1	hypothetical protein	-
KU667_RS26165 (KU667_26075)	50722..51192	-	471	WP_016528981.1	RES family NAD+ phosphorylase	-
KU667_RS26170 (KU667_26080)	51189..51632	-	444	WP_004026357.1	DUF2384 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..174986	174986
	-	flank	IS/Tn	-	-	47394..48119	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T207949 WP_021312536.1 NZ_CP077815:c46378-45893 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T207949 NZ_CP103546:c62751-62599 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT207949 WP_003026799.1 NZ_CP077815:c46632-46366 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT207949 NZ_CP103546:62801-62863 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66