Detailed information of TA system TA207947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 5095449..5096185
• Replicon: chromosome
• Accession: NZ_CP077814
• Organism: Klebsiella pneumoniae strain FF1076

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KU667_RS24820
• Protein ID: WP_251341019.1
• Coordinates: 5095449..5095931 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: KU667_RS24825
• Protein ID: WP_251341020.1
• Coordinates: 5095919..5096185 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU667_RS24795 (KU667_24695)	5090852..5091430	+	579	WP_032432061.1	type 1 fimbrial protein	-
KU667_RS24800 (KU667_24700)	5091442..5092386	+	945	WP_077256325.1	fimbrial protein	-
KU667_RS24805 (KU667_24705)	5092600..5093199	+	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
KU667_RS24810 (KU667_24710)	5093648..5093839	+	192	WP_048293752.1	hypothetical protein	-
KU667_RS24815 (KU667_24715)	5093892..5095238	-	1347	WP_251341018.1	ISNCY family transposase	-
KU667_RS24820 (KU667_24720)	5095449..5095931	-	483	WP_251341019.1	GNAT family N-acetyltransferase	Toxin
KU667_RS24825 (KU667_24725)	5095919..5096185	-	267	WP_251341020.1	DUF1778 domain-containing protein	Antitoxin
KU667_RS24830 (KU667_24730)	5096498..5097061	-	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
KU667_RS24835 (KU667_24735)	5097071..5097703	-	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
KU667_RS24840 (KU667_24740)	5097721..5098725	-	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
KU667_RS24845 (KU667_24750)	5098709..5099329	-	621	Protein_4856	dihydroxyacetone kinase subunit DhaL	-
KU667_RS24850 (KU667_24755)	5099400..5100539	-	1140	WP_040025615.1	mannitol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5083790..5128240	44450
	-	flank	IS/Tn	-	-	5093892..5095250	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17351.91 Da
• Isoelectric Point: 9.1200

>T207947 WP_251341019.1 NZ_CP077814:c5095931-5095449 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T207947 NZ_CP103545:77382-77780 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10132.62 Da
• Isoelectric Point: 4.5256

>AT207947 WP_251341020.1 NZ_CP077814:c5096185-5095919 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPLWDV

Nucleotide

• Length: 267 bp

>AT207947 NZ_CP103545:77155-77382 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA