Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1912385..1913195 Replicon chromosome
Accession NZ_CP077814
Organism Klebsiella pneumoniae strain FF1076

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KU667_RS09270 Protein ID WP_004178461.1
Coordinates 1912662..1913195 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KU667_RS09265 Protein ID WP_002887278.1
Coordinates 1912385..1912651 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU667_RS09245 (KU667_09240) 1908256..1908600 - 345 WP_048266266.1 cation efflux system protein CusF -
KU667_RS09250 (KU667_09245) 1908618..1910003 - 1386 WP_134909742.1 efflux transporter outer membrane subunit -
KU667_RS09255 (KU667_09250) 1910176..1910859 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KU667_RS09260 (KU667_09255) 1910849..1912282 + 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
KU667_RS09265 (KU667_09260) 1912385..1912651 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KU667_RS09270 (KU667_09265) 1912662..1913195 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KU667_RS09275 (KU667_09270) 1913243..1914364 - 1122 WP_064153030.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T207935 WP_004178461.1 NZ_CP077814:1912662-1913195 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T207935 NZ_CP103544:2702894-2703001 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGACGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT207935 WP_002887278.1 NZ_CP077814:1912385-1912651 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT207935 NZ_CP103544:c2702847-2702781 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References