Detailed information of TA system TA207925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3903091..3903716
• Replicon: chromosome
• Accession: NZ_CP077812
• Organism: Klebsiella pneumoniae strain FF1083

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: KU672_RS18940
• Protein ID: WP_002882817.1
• Coordinates: 3903091..3903474 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KU672_RS18945
• Protein ID: WP_074185945.1
• Coordinates: 3903474..3903716 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU672_RS18925 (3900457)	3900457..3901359	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
KU672_RS18930 (3901356)	3901356..3901991	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
KU672_RS18935 (3901988)	3901988..3902917	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
KU672_RS18940 (3903091)	3903091..3903474	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
KU672_RS18945 (3903474)	3903474..3903716	-	243	WP_074185945.1	CopG family transcriptional regulator	Antitoxin
KU672_RS18950 (3903921)	3903921..3904837	+	917	Protein_3696	alpha/beta hydrolase	-
KU672_RS18955 (3904851)	3904851..3905792	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
KU672_RS18960 (3905837)	3905837..3906274	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
KU672_RS18965 (3906271)	3906271..3907131	-	861	WP_004146232.1	virulence factor BrkB family protein	-
KU672_RS18970 (3907125)	3907125..3907724	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T207925 WP_002882817.1 NZ_CP077812:c3903474-3903091 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T207925 NZ_CP103544:c595029-594565 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9537.74 Da
• Isoelectric Point: 4.3509

>AT207925 WP_074185945.1 NZ_CP077812:c3903716-3903474 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGTEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT207925 NZ_CP103544:c595364-595029 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8