Detailed information of TA system TA207911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 541750..542340
• Replicon: chromosome
• Accession: NZ_CP077812
• Organism: Klebsiella pneumoniae strain FF1083

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KU672_RS02425
• Protein ID: WP_021466317.1
• Coordinates: 542008..542340 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: KU672_RS02420
• Protein ID: WP_000288812.1
• Coordinates: 541750..542007 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU672_RS02405 (539093)	539093..540355	-	1263	WP_200549280.1	hypothetical protein	-
KU672_RS02410 (540740)	540740..541212	+	473	Protein_468	hypothetical protein	-
KU672_RS02415 (541209)	541209..541415	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
KU672_RS02420 (541750)	541750..542007	+	258	WP_000288812.1	antitoxin	Antitoxin
KU672_RS02425 (542008)	542008..542340	+	333	WP_021466317.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU672_RS02435 (542663)	542663..544099	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
KU672_RS02445 (544465)	544465..545919	-	1455	WP_004148975.1	AMP nucleosidase	-
KU672_RS02450 (546049)	546049..546294	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rcsA	541209..619649	78440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.82 Da
• Isoelectric Point: 10.4722

>T207911 WP_021466317.1 NZ_CP077812:542008-542340 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDAVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T207911 NZ_CP103540:c2406926-2406750 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT207911 WP_000288812.1 NZ_CP077812:541750-542007 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT207911 NZ_CP103540:c2406704-2406288 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0