Detailed information of TA system TA207909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 30698..31220
• Replicon: plasmid pFF904_1
• Accession: NZ_CP077811
• Organism: Klebsiella pneumoniae strain FF904

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: KU662_RS25330
• Protein ID: WP_004187019.1
• Coordinates: 30698..30982 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: KU662_RS25335
• Protein ID: WP_004187025.1
• Coordinates: 30972..31220 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU662_RS25305	25918..26118	-	201	WP_251336595.1	conjugative transfer protein MobI(A/C)	-
KU662_RS25310	26160..26381	-	222	WP_251336594.1	hypothetical protein	-
KU662_RS25315 (KU662_25250)	27924..28337	+	414	WP_004187013.1	hypothetical protein	-
KU662_RS25320 (KU662_25260)	28994..29245	+	252	Protein_26	conjugal transfer protein TraH	-
KU662_RS25325 (KU662_25265)	29327..30682	+	1356	WP_004187017.1	ISNCY family transposase	-
KU662_RS25330 (KU662_25270)	30698..30982	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KU662_RS25335 (KU662_25275)	30972..31220	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
KU662_RS25340 (KU662_25280)	31477..32445	-	969	WP_019704591.1	IS5-like element IS903B family transposase	-
KU662_RS25345 (KU662_25285)	32478..32783	-	306	Protein_31	arsenate reductase (glutaredoxin)	-
KU662_RS25350 (KU662_25290)	32796..34085	-	1290	WP_004187067.1	arsenic transporter	-
KU662_RS25355 (KU662_25295)	34130..34450	-	321	WP_026005941.1	transcriptional regulator	-
KU662_RS25360 (KU662_25300)	34536..34921	+	386	Protein_34	NAD(P)H-dependent oxidoreductase	-
KU662_RS25365 (KU662_25305)	35190..36179	-	990	WP_004187061.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..140918	140918
	-	flank	IS/Tn	-	-	31477..32445	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T207909 WP_004187019.1 NZ_CP077811:c30982-30698 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T207909 NZ_CP103540:2007601-2007704 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT207909 WP_004187025.1 NZ_CP077811:c31220-30972 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT207909 NZ_CP103540:c2007742-2007471 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4