Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2715403..2716022 Replicon chromosome
Accession NZ_CP077810
Organism Klebsiella pneumoniae strain FF904

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU662_RS13255 Protein ID WP_002892050.1
Coordinates 2715804..2716022 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU662_RS13250 Protein ID WP_002892066.1
Coordinates 2715403..2715777 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU662_RS13240 (2710555) 2710555..2711748 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU662_RS13245 (2711771) 2711771..2714917 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KU662_RS13250 (2715403) 2715403..2715777 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU662_RS13255 (2715804) 2715804..2716022 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU662_RS13260 (2716181) 2716181..2716747 + 567 WP_002892030.1 maltose O-acetyltransferase -
KU662_RS13265 (2716719) 2716719..2716859 - 141 WP_004147370.1 hypothetical protein -
KU662_RS13270 (2716880) 2716880..2717350 + 471 WP_002892026.1 YlaC family protein -
KU662_RS13275 (2717325) 2717325..2718776 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KU662_RS13280 (2718877) 2718877..2719575 + 699 WP_032416783.1 GNAT family protein -
KU662_RS13285 (2719572) 2719572..2719712 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU662_RS13290 (2719712) 2719712..2719975 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T207900 WP_002892050.1 NZ_CP077810:2715804-2716022 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T207900 NZ_CP103537:28973-29254 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT207900 WP_002892066.1 NZ_CP077810:2715403-2715777 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT207900 NZ_CP103537:28732-28983 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References