Detailed information of TA system TA207888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3818924..3819660
• Replicon: chromosome
• Accession: NZ_CP077809
• Organism: Klebsiella pneumoniae strain FF923

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KU666_RS18620
• Protein ID: WP_003026803.1
• Coordinates: 3818924..3819406 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KU666_RS18625
• Protein ID: WP_003026799.1
• Coordinates: 3819394..3819660 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU666_RS18600 (3815473)	3815473..3816213	+	741	WP_048978466.1	molecular chaperone	-
KU666_RS18605 (3816217)	3816217..3816795	+	579	WP_032432061.1	type 1 fimbrial protein	-
KU666_RS18610 (3816807)	3816807..3817751	+	945	WP_077254249.1	fimbrial protein	-
KU666_RS18615 (3817963)	3817963..3818562	+	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
KU666_RS18620 (3818924)	3818924..3819406	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KU666_RS18625 (3819394)	3819394..3819660	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KU666_RS18630 (3819836)	3819836..3820090	-	255	WP_004152108.1	hypothetical protein	-
KU666_RS18635 (3820166)	3820166..3820423	-	258	WP_004152107.1	hypothetical protein	-
KU666_RS18640 (3820472)	3820472..3820675	-	204	WP_004152106.1	HHA domain-containing protein	-
KU666_RS18645 (3820709)	3820709..3821077	-	369	WP_004152105.1	hypothetical protein	-
KU666_RS18650 (3821121)	3821121..3821615	-	495	WP_004152104.1	DNA-binding protein	-
KU666_RS18655 (3821646)	3821646..3822221	-	576	WP_004152103.1	hypothetical protein	-
KU666_RS18660 (3822209)	3822209..3822478	-	270	WP_004152102.1	hypothetical protein	-
KU666_RS18665 (3822836)	3822836..3823186	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KU666_RS18670 (3823236)	3823236..3823598	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3809155..3869713	60558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T207888 WP_003026803.1 NZ_CP077809:c3819406-3818924 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T207888 NZ_CP103533:1879667-1879918 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT207888 WP_003026799.1 NZ_CP077809:c3819660-3819394 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT207888 NZ_CP103533:1879915-1880169 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66