Detailed information of TA system TA207887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3470625..3471215
• Replicon: chromosome
• Accession: NZ_CP077809
• Organism: Klebsiella pneumoniae strain FF923

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KU666_RS17085
• Protein ID: WP_023341911.1
• Coordinates: 3470625..3470957 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: KU666_RS17090
• Protein ID: WP_000288812.1
• Coordinates: 3470958..3471215 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU666_RS17060 (3466672)	3466672..3466917	+	246	WP_023284006.1	signal transduction protein PmrD	-
KU666_RS17065 (3467047)	3467047..3468501	+	1455	WP_004148975.1	AMP nucleosidase	-
KU666_RS17075 (3468867)	3468867..3470303	-	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
KU666_RS17085 (3470625)	3470625..3470957	-	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU666_RS17090 (3470958)	3470958..3471215	-	258	WP_000288812.1	antitoxin	Antitoxin
KU666_RS17095 (3471550)	3471550..3471756	-	207	WP_022615589.1	helix-turn-helix transcriptional regulator	-
KU666_RS17100 (3471753)	3471753..3472214	-	462	WP_040210976.1	hypothetical protein	-
KU666_RS17105 (3472842)	3472842..3473258	+	417	WP_124047552.1	hypothetical protein	-
KU666_RS17110 (3473266)	3473266..3474615	+	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T207887 WP_023341911.1 NZ_CP077809:c3470957-3470625 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T207887 NZ_CP103533:1391218-1391616 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT207887 WP_000288812.1 NZ_CP077809:c3471215-3470958 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT207887 NZ_CP103533:1390991-1391218 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0