Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 635186..635996 Replicon chromosome
Accession NZ_CP077809
Organism Klebsiella pneumoniae strain FF923

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KU666_RS03020 Protein ID WP_004178461.1
Coordinates 635463..635996 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KU666_RS03015 Protein ID WP_002887278.1
Coordinates 635186..635452 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU666_RS02995 (631070) 631070..631402 - 333 WP_025368487.1 cation efflux system protein CusF -
KU666_RS03000 (631420) 631420..632805 - 1386 WP_221013835.1 efflux transporter outer membrane subunit -
KU666_RS03005 (632977) 632977..633660 + 684 WP_020804437.1 copper response regulator transcription factor CusR -
KU666_RS03010 (633650) 633650..635083 + 1434 WP_021463709.1 Cu(+)/Ag(+) sensor histidine kinase -
KU666_RS03015 (635186) 635186..635452 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KU666_RS03020 (635463) 635463..635996 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KU666_RS03025 (636044) 636044..637165 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T207876 WP_004178461.1 NZ_CP077809:635463-635996 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T207876 NZ_CP103530:67664-67813 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT207876 WP_002887278.1 NZ_CP077809:635186-635452 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT207876 NZ_CP103530:c67620-67559 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References