Detailed information of TA system TA207872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 124448..125091
• Replicon: plasmid pFF979_2
• Accession: NZ_CP077808
• Organism: Klebsiella pneumoniae strain FF979

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KU665_RS26425
• Protein ID: WP_251336356.1
• Coordinates: 124448..124864 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: KU665_RS26430
• Protein ID: WP_001261276.1
• Coordinates: 124861..125091 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU665_RS26385 (KU665_26315)	120706..120960	-	255	WP_094833286.1	hypothetical protein	-
KU665_RS26390 (KU665_26320)	120964..121176	-	213	Protein_132	hypothetical protein	-
KU665_RS26395 (KU665_26325)	121240..121470	-	231	WP_251336352.1	hypothetical protein	-
KU665_RS26400 (KU665_26330)	121484..121687	-	204	WP_012540085.1	HHA domain-containing protein	-
KU665_RS26405 (KU665_26335)	121737..122231	-	495	WP_251336353.1	DNA-binding protein	-
KU665_RS26410 (KU665_26340)	122262..122834	-	573	WP_095858476.1	hypothetical protein	-
KU665_RS26415 (KU665_26345)	122831..123079	-	249	WP_251336354.1	hypothetical protein	-
KU665_RS26420 (KU665_26350)	123573..124196	+	624	WP_251336355.1	TetR/AcrR family transcriptional regulator	-
KU665_RS26425 (KU665_26355)	124448..124864	-	417	WP_251336356.1	type II toxin-antitoxin system VapC family toxin	Toxin
KU665_RS26430 (KU665_26360)	124861..125091	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KU665_RS26435 (KU665_26365)	125445..127052	-	1608	WP_251336357.1	prolyl oligopeptidase family serine peptidase	-
KU665_RS26440 (KU665_26370)	127031..128215	-	1185	WP_251336358.1	MFS transporter	-
KU665_RS26445 (KU665_26375)	128208..129695	-	1488	WP_251336359.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..135334	135334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15036.55 Da
• Isoelectric Point: 9.2957

>T207872 WP_251336356.1 NZ_CP077808:c124864-124448 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIATGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T207872 NZ_CP103529:c4280304-4280017 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT207872 WP_001261276.1 NZ_CP077808:c125091-124861 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT207872 NZ_CP103529:c4280030-4279728 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023